Islet and Physiology Core

IU Indianapolis

Carmella Evans-Molina, MD, PhD

Jason Spaeth, PhD

https://iu.ilab.agilent.com/service_center/show_external/5519/islet_physiology_core

The Islet and Physiology Core of the Center for Diabetes and Metabolic Diseases provides investigators with access to high quality rodent pancreatic islets and pancreatic slices.  The Core provides services for islet transplantation and will assist investigators who wish to perform immunohistochemistry, immunofluorescence, and/or analysis of beta cell mass on whole pancreata from mouse and rat models.  We are also pleased to offer several services for rodent metabolic characterization, including performance of insulin and glucose tolerance testing and analysis of body composition.  We are able to facilitate metabolic cage analysis using the TSE System and assist with data analysis. In addition, the Core provides human islet phenotyping services and can assist investigators in finding appropriate sources of human pancreas tissue for their studies.  The Islet and Physiology Core functions as part of the Indiana Diabetes Research Center and the Indiana University School of Medicine Center for Diabetes and Metabolic Diseases, to foster new knowledge, support training, and promote basic and translational research in diabetes and related metabolic disorders and their complications.

To cite use of this core, please use the following verbiage: “This research was performed with support from the Islet & Physiology Core of the NIH P30-funded Indiana Diabetes Research Center (P30DK097512)”

• Islet Core Policies

• High quality rodent (mouse and rat) islets.

• Mouse islet transplantation under the kidney capsule.

• Rodent tissue collection.

• Pancreatic staining, including endocrine cell staining and endocrine cell mass/area analysis.  Services under this category include beta and alpha cell mass/area analysis.

• Mouse metabolic characterization, including glucose/insulin tolerance tests, body composition measurements, and facilitate metabolic cage testing and analysis.

• Islet physiology including glucose stimulated insulin secretion, perifusion analysis and intracellular calcium analysis in fixed cells

• Extracellular vesicle and exosome analysis services.

• Rapid scanning of slides using a Keyence system for imaging in fluorescence, brightfield, and phase contrast with automated XYZ stitching of large histology sections.

• Consultation and training in islet maintenance, experimentation, rodent diet studies, and data interpretation.

• Access to pancreatic slices

• Kara Orr : ksbennin@iu.edu 317-278-8940